The 2012 study by Akhavein that described allergic mastocytic enterocolitis also performed biopsies on the stomach of patients with a history of atopic/allergic disease were biopsied. Mast cells were identified using an antibody to CD117, the CKIT receptor found on the surface of all mast cells. The cells were counted in only 1 hpf. On average, there were 39 mast cells/hpf with a range of 16-82 mast cells/hpf. These cells were also scattered and not clustered. See Table 13 for details.
|Table 13: Mast cell count in stomach of patients with GI pain and dysmotility and a history of allergic disease|
|Akhavein AM, et al. Allergic mastocytic gastroenteritis and colitis: An unexplained etiology in chronic abdominal pain and gastrointestinal dysmotility. Gastroenterology Research and Practice (2012): Article ID 950582.|
|Stomach||Study group: atopic/allergic history with abdominal pain and GI dysmotility||Control group A:
No control group
|Control group B:
No control group
|39 mast cells/hpf||16-82 mast cells/hpf||N/A||N/A||N/A||N/A|
|Diffuse, scattered cells, no clusters.|
A 2015 publication evaluated the mast cell count in patients with chronic diarrhea for unknown reasons. Mast cells were quantified using an antibody to CD117. Cells were only counted in 1 hpf in the portion of the slide with the most mast cells. The healthy control group averaged 24 mast cells/hpf, while the study group with chronic diarrhea averaged 31 mast cells/hpf. See Table 14 for details.
|Table 14: Mast cell count in colon of patients with chronic diarrhea|
|Sethi A, et al. Performing colonic mast cell counts in patients with chronic diarrhea of unknown etiology has limited diagnostic use. Arch Pathol Lab Med 2015; 139 (2): 225-232.|
|Microscopy method: 400x magnification, mast cells counted in 1 hpf|
|Visualization: CD117 and tryptase (IHC)|
|Sample type||Study group: Chronic diarrhea||Control group A:
|Control group B:
No control group
|31 mast cells/hpf||24-34 mast cells/hpf||24 mast cells/hpf||22-27 mast cells/hpf||N/A||N/A|
|Diffuse scattered cells, no clusters.||Diffuse scattered cells, no clusters.|
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